Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required to suppress ice formation. Recently, we described a mathematical strategy for identifying minimally toxic CPA equilibration procedures based on the minimization of a toxicity cost function. Here we provide direct experimental support for the feasibility of these methods when applied to adherent endothelial cells.
The Cryonics Institute's Standby Check-In app for Android devices is now available on Google Play for 99¢.
Why Businesses Embrace Machine Learning [Excerpt]
The best algorithms determine who wins and loses in a digital economy that thrives on steering the consumer toward an opportune click
By Pedro Domingos | October 29, 2015
Why is Google worth so much more than Yahoo? They both make their money from showing ads on the web, and they’re both top destinations. Both use auctions to sell ads and machine learning to predict how likely a user is to click on an ad (the higher the probability, the more valuable the ad). But Google’s learning algorithms are much better than Yahoo’s. This is not the only reason for the difference in their market caps, of course, but it’s a big one. Every predicted click that doesn’t happen is a wasted opportunity for the advertiser and lost revenue for the website. With Google’s annual revenue of $50 billion, every 1 percent improvement in click prediction potentially means another half billion dollars in the bank, every year, for the company. No wonder Google is a big fan of machine learning, and Yahoo and others are trying hard to catch up.