The Cryonics Institute’s 128th Patient
Cryonics Institute Case Report for Patient Number 128
CI patient #128 was a 32 year old female who died in Arizona on January 4th, 2015. The cause of death was Acute Myelogenous Leukemia. The patient was a CI member at the time of her death.
The next of kin had contracted with a local funeral director to provide initial cooling and transport services. The patient died at approximately 6:30am on January 4th, 2015. The patient was picked up by the cooperating funeral director and packed in water ice and was taken to the cooperating funeral directors facility, where she was maintained in water ice. Heparin was not administered.
The patient arrived at the CI facility, packed in water ice at approximately 7:30pm on the 6th of January. Jim and Sara Walsh, CIs local cooperating funeral directors, were both present for the perfusion and the perfusion began at 7:45pm. No clotting of the blood was observed during the perfusion. The body and head were both perfused and a flow rate of 1.66 liters per minute was maintained at 118mm pressure.
The perfusion was completed at 8:45pm. During the perfusion there were 2 liters of 10% Eg solution used, 4 liters of 30 % Eg solution used and 30 liters of 70% VM1 solutions used. The final refractive index of the effluents exiting the right jugular vein was 1.4168. The final refractive index of the effluents exiting the left jugular vein was 1.40. The perfusion was terminated before the desired refractive index of 1.424 was achieved because the flow from the jugular veins from the head decreased to the point of almost stopping and edema was starting to appear in the face. No edema was noted in the body and bronzing of the skin and dehydration was noted all the way to the toes and finger tips.
The patient was then placed in the computer controlled cooling chamber to cool to liquid nitrogen temperature. The human vitrification program was selected and the time needed to cool the patient to liquid nitrogen temperature was five days and 14 hours. The patient was then placed in a cryostat for long-term cryonic storage.
Comments: The state of Arizona will not issue any paperwork for transit on the weekends, and this caused a delay in getting the patient to CI. The perfusion went quite well, especially considering that there was about 60 hours of cold ischemia. The reason for this could be that the patient was quickly packed in water ice and the patient was thin, which would allow the patient to cool down faster. Less amounts of the 10% and 20% solutions were used in the perfusion because past experiences have shown that we can obtain better saturation of the tissue with vitrification solution, with less edema, if we use less amounts of the lower concentrations of solutions and advance quicker to the 70% solution when there is more than 24 hours of cold ischemia. The reasons for no clotting of the blood, though no heparin was administered, are unclear. It may be possible that the medications the patient was receiving as treatment for Leukemia helped to prevent coagulation, but at the time the report was written the types of medication the patient was receiving are unknown.